Stepwise Culture of Human Adult Stem Cells by Pranela Rameshwar Hardcover Book

Publisher Description

This volume on stepwise techniques to culture adult human stem cells begins with the basic methods to culture adult stem cells followed by related methods to supplement stem cell culture. This and future volumes of techniques will allow laboratories across scientific entities – academic and commercial – to adapt the described methods for similar methods with cells from other species. The standardized techniques will allow across the board interpretation of other technologies such as Omics studies. These methods can be a starting platform for culturing stem cells even in laboratories that might not be familiar with the techniques.Scientific and early clinical trials strongly favor the development of translational studies with adult stem cells. A major impediment to progress in the field of regenerative medicine is a lack of standardized methods to culture specific stem cells. Such documented methods would allow for scientific rigor and reproducibility to enhance efficient and safe translation of stem cells to patients. This book fills that gap.

Table of Contents

Introduction: Core Techniques in Stem Cell Culture 1. Isolation of Hematopoietic Stem Cells 2. Isolation Protocol for CD133+ and CD34+ Very Small Embryonic Like Cells (VSEL) from Human Umbilical Cord Blood3. Isolation and Expansion of Mesenchymal Stem Cells from the Adult Bone Marrow Aspirate4. Manual Isolation and Expansion of Adipose Derived Stem Cells from Adipose Tissue5. Isolating Compact Bone-derived Mesenchymal Stem Cells from Rodent and Rabbit Femurs 6. Isolating Dental Pulp Stem Cells7. A Murine System for Somatic Cellular Reprogramming into Induced Pluripotent Stem Cells8. Isolation and Expansion Of Placenta Stem Cells (P-MSCs) 9. Expanding Human Mesenchymal Stem Cells from the Umbilical Cord 10. Laboratory Isolation of Leukemia Cancer Stem Cell11. Isolation and Characterization of Cancer Stem cells from Glioblastoma Multiforme and Breast Cancer Cell Lines12. Bone Marrow Aspirate for Isolation of Mesenchymal Stem Cells13. Culturing Human Bone Marrow Stromal Cells14. Isolating Mononuclear Cells by Ficoll-Hypaque Density Gradient15. Phenotypic and Multipotent Characterization of Bone Marrow-derived Mesenchymal Stem Cells16. Cryopreservation of Relatively Small Number of Stem Cells17. Cryopreservation of Mobilized Peripheral Blood Stem Cells18. Manual Cell Count

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